Cell Culture Isolation of Piscine Nodavirus (Betanodavirus) in Fish-Rearing Seawater
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چکیده
منابع مشابه
Cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass, Dicentrarchus labrax.
A virus causing a vacuolating encephalopathy and retinopathy in juvenile sea bass, Dicentrarchus labrax, was isolated from brain tissue in a fish cell line (SSN-1) derived from striped snakehead, Channa striatus. The isometric, non-enveloped, 30 nm diameter virus particles were resistant to pH 2-9 and heating at 56 degrees C for 30 min. Infectious particles had a buoyant density of approximatel...
متن کاملSusceptibility of the fish cell line SAF-1 to betanodavirus.
Betanodavirus infections of fish, generally known as viral nervous necrosis or viral encephalopathy and retinopathy, are a serious threat to marine fish farming throughout the world (Munday, Kwang & Moody 2002). The culture of betanodavirus has proved complex because most commonly available fish cell lines do not allow the growth of these viruses, as reviewed by Munday & Nakai (1997). The diffi...
متن کاملFish nodavirus lytic cycle and semipermissive expression in mammalian and fish cell cultures.
In this study, Dicentrarchus labrax encephalitis virus (DlEV), which causes sea bass encephalitis, was propagated in cell culture, thus allowing study of its lytic cycle. DlEV infection of mammalian and fish cells induced different patterns of expression of capsid proteins, which were assembled as virus-like particles, accumulating in the cytoplasm either as diffuse masses or in vesicles, as sh...
متن کاملIsolation in Cell Culture
The aetiology of inflammatory bowel disease is at present obscure, but there is evidence that transmissible agents such as bacteria or viruses may be associated with this condition.' 4 Chlamydia trachomatis agents are known to infect the rectum5 6 and lymphogranuloma venereum (LGV) serotypes can cause chronic inflammation with fistula formation similar to manifestations of Crohn's disease. Prev...
متن کاملCrystal Structures of a Piscine Betanodavirus: Mechanisms of Capsid Assembly and Viral Infection
Betanodaviruses cause massive mortality in marine fish species with viral nervous necrosis. The structure of a T = 3 Grouper nervous necrosis virus-like particle (GNNV-LP) is determined by the ab initio method with non-crystallographic symmetry averaging at 3.6 Å resolution. Each capsid protein (CP) shows three major domains: (i) the N-terminal arm, an inter-subunit extension at the inner surfa...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2016
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.03834-15